Saliva Genotyping Using the 96.96 IFC with SNP Type Assays

Abstract

Single nucleotide polymorphisms (SNPs) are highly useful in genotyping, as they are the most common genetic variants in the human genome and are unique to each individual. Saliva SNP testing is a new undertaking, and exploration and optimization of saliva sample parameters are required to authenticate the composition of a saliva sample and link it to a specific person. SNP studies were improved with the use of microfluidics PCR and SNP assay technologies and run with a 96.96 dynamic array IFC and Biomark X PCR machine. Our study consisted of numerous trials with a variety of parameters to observe differences in confidence and call rates of SNPs present in a given sample. Various conditions were studied including volume, contamination, additives, age, and temperature. The results vary depending on the concentration strength of additives in a definite volume. Saliva drug testing has increased in the last few years compared to urine drug testing due to the versatility in sample collection, decreased invalid tests, and enhanced detection of recent drug use. The objective of this study is to combine saliva SNPs with saliva drug testing to eliminate illegal interference, promoting accurate drug examinations.